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  Advanced Solutions and Technologies for Life Sciences Friday, October 31, 2014

 
                XNAPS Endofree Plasmid Minispin Kit                      Manual(PDF)
               XNAPS Plasmid Endofree Flexspin Kit                      Manual(PDF)



                                                                       XNAPS Plasmid Endofree  Minispin Kit

Catalog No.:  NP1002A/ NP1002B

Application:  Transfection grade plasmid or cosmid purification from small volume (1-5 ml) of bacterial culture.

Introduction 

XNAPS plasmid endofree minispin kit provides a fast and efficient method for purifying transfection grade plasmid from small volumes of bacterial culture(1-3 ml). The system integrates a modified alkaline lysis procedure, removal of endotoxin from plasmid, and an innovative chromatographic absorbent resin which preferentially binds DNA in a system. The novel procedure provided by the kit eliminates many disadvantages, such as time-consuming, toxic components, low yield, from other commercial kits.

The purified transfection grade plasmid DNA is ready to use in various downstream applications, such as PCR, restriction enzyme digestion, labeling, sequencing, transformation, transfection, and in vitro transcription/translation.

Key Features

  • Endotoxin free : <0.1EU/ μg of purified plasmid.
  • Speed : All the processing steps can be completed in 30 minutes.
  • High yield and purity : Up to 30 μg of high-copy plasmid with OD 260/OD 280 between 1.8-2.0.can be extracted from 3 ml bacterial culture
  • Safety for handling, shipping and storage : No phenol extraction, no ethanol precipitation, no toxic chaotropic salts are necessary.
    

Comparison of Renogen endofree plasmid minispin kit with Qiagen plasmid mini kit

 

Purity

processing Time

Equipments and reagents provided by user

Ethanol precipitation

Price(USD/prep)

Renogen XNAPS Endofree plasmid minispin kit—transfection grade (Cat. No. NP1002B)

OD 260/OD 280=1.8-2.0

Endotoxin <0.1 EU/µg

<30 min

  • Standard equipment for growing and harvesting bacteria
  • Microcentrifuge
  • 1.5 ml centrifuge tubes
  • Ice
  • ethanol

No

2.80

Qiagen Plasmid Mini Kit-- transfection grade (cat. No. 12125)

OD 260/OD 280: unknown

Endotoxin: unknown

>120min

  • Standard equipment for growing and harvesting bacteria
  • Microcentrifuge
  • 1.5 ml or 2 ml centrifuge tubes
  • QIArack or equivalent holder
  • Ice
  • Isopropanol
  • 70% ethanol
  • Plasmid resuspension buffer

 

Yes

6.29

    

                                             


                                                                       XNPAS Plasmid Endofree Flexspin Kit
Catalog No.:  NP1003

Application:  Transfection grade plasmid or cosmid purification from variable amount of  (1- 150 ml) of bacterial culture.

Introduction 

The unique and innovative XNAPS plasmid endofree flexspin kit provides a highly flexible, fast, simple, and efficient method for isolating transfection grade plasmid from mini (1 ml) to maxi (150 ml) volumes of bacterial culture. The system integrates a modified alkaline lysis procedure, removal of endotoxin from plasmid, and an innovative chromatographic absorbent resin which preferentially binds DNA in a whole system. The kit combines the rapidity and simplicity of spin column method with the flexibility of batch processing for plasmid DNA purification. The novel procedure provided by the kit eliminates many disadvantages, such as time-consuming, toxic components, low yield, from other commercial kits.

The purified transfection grade plasmid DNA is ready to use in various downstream applications, such as PCR, restriction enzyme digestion, labeling, sequencing, transformation, transfection, and in vitro transcription/translation.

Key Features

  • Endotoxin free : <0.1EU/ μg of purified plasmid.
  • Speed : Depending on the culture volume, all the processing steps can be completed within 30-60 minutes.
  • High flexibility : The kit can be used efficiently from small culture volume (1-5 ml) to midi volume (50 ml), even to maxi volume(150 ml).
  • High yield and purity : Up to 30-500 μg of high-copy plasmid with a ratio of OD 260/OD 280 > 1.8 can be extracted from 3-150 ml bacterial culture.
  • Safety for handling, shipping and storage : No phenol extraction, no ethanol precipitation, no toxic chaotropic salts are necessary.
  • The kit contains enough reagents for 100 minipreps, or 25 midipreps, or 10 maxipreps.
 







                              
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